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rdf:type |
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lifeskim:mentions |
umls-concept:C0018183,
umls-concept:C0018270,
umls-concept:C0020410,
umls-concept:C0021764,
umls-concept:C0079460,
umls-concept:C0086418,
umls-concept:C0439158,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1707455,
umls-concept:C2911692
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pubmed:issue |
19
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pubmed:dateCreated |
1987-11-4
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pubmed:abstractText |
Supernatants of COS-1 cells transfected with gibbon cDNA encoding interleukin 3 (IL-3) with homology to sequences for human IL-3 were tested for ability to promote growth of various human hemopoietic progenitors. The effect of these supernatants as a source of recombinant IL-3 was compared to that of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) as well as to that of medium conditioned by phytohemagglutinin-stimulated leukocytes. The frequency of multilineage colonies, erythroid bursts, and megakaryocyte colonies in cultures containing the COS-1 cell supernatant was equivalent to the frequency observed in the controls and significantly higher than found in cultures plated with recombinant GM-CSF. G-CSF did not support the formation of multilineage colonies, erythroid bursts, and megakaryocyte colonies. In contrast, growth of granulocyte-macrophage colonies was best supported with GM-CSF, while recombinant IL-3 yielded colonies at lower or at best equivalent frequency. The simultaneous addition of higher concentrations of GM-CSF to cultures containing IL-3 in optimal amounts did not enhance the formation of multilineage colonies, erythroid bursts, and megakaryocyte colonies. However, the frequency of such colonies and bursts increased with GM-CSF when cultures were plated with suboptimal concentrations of IL-3. Growth of colonies within the granulocyte-macrophage lineage is optimally supported by GM-CSF and does not increase with further addition of IL-3.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-2420009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-2996129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3484428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3484805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3486413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3489530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3865178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3876355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3877981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3923623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3925454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3930522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-3979471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-4518063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-5056784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-6234038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-6490728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3498941-6802859
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6765-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3498941-Animals,
pubmed-meshheading:3498941-Bone Marrow Cells,
pubmed-meshheading:3498941-Cell Division,
pubmed-meshheading:3498941-Cells, Cultured,
pubmed-meshheading:3498941-Granulocytes,
pubmed-meshheading:3498941-Hematopoietic Stem Cells,
pubmed-meshheading:3498941-Humans,
pubmed-meshheading:3498941-Hylobates,
pubmed-meshheading:3498941-Interleukin-2,
pubmed-meshheading:3498941-Interleukin-3,
pubmed-meshheading:3498941-Kinetics,
pubmed-meshheading:3498941-Macrophages,
pubmed-meshheading:3498941-Recombinant Proteins
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pubmed:year |
1987
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pubmed:articleTitle |
Growth of human hemopoietic colonies in response to recombinant gibbon interleukin 3: comparison with human recombinant granulocyte and granulocyte-macrophage colony-stimulating factor.
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pubmed:affiliation |
Ontario Cancer Institute, Toronto, Canada.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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