Linked Life Data

3494698

Source:http://linkedlifedata.com/resource/pubmed/id/3494698

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1987-5-28
pubmed:abstractText
Double stranded DNA from calf thymus was irradiated in the presence of bovine serum albumin with a ratio of 1:10 in weight under aerobic and anaerobic conditions. The irradiated biomolecules were separated by gel filtration on Sepharose Cl-2B with and without sodium dodecylsulphate. By scavenging OH-radicals the protein protects DNA, but in contrast to radiolysis of DNA in phosphate buffer, in the presence of serum albumin oxygen enhances the degradation of DNA. Radiolysis under N2 leads to crosslinking of serum albumin to DNA with higher yields at pH 5 than at pH 7. Oxygen largely prevents crosslinking.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0020-7616
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
455-66
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
The effect of a protein on the radiolysis of DNA studied by gel filtration.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't