Linked Life Data

3487878

Source:http://linkedlifedata.com/resource/pubmed/id/3487878

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-8-21
pubmed:abstractText
A rapid and easily performed colorimetric method has been adapted for the detection of the Pasteurella haemolytica leucotoxin using the lysis of bovine leucocyte BL-3 cells (a bovine lymphoid cell line obtained from Dr. G. Theilen, University of California, Davis). The method involves incubating BL-3 cells in the presence of P. haemolytica culture supernatant and assessing cell survival at the end of the assay by staining remaining BL-3 cells with the dye neutral red. Solubilization of the cells with concomitant dye release allows the assay to be read by measuring optical density (540 nm) with an automated spectrophotometer. This assay can also be used for the determination of neutralizing antiserum titres.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-1135
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Automated colorimetric assay for the detection of Pasteurella haemolytica leucotoxin.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't