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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1987-11-4
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pubmed:databankReference |
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pubmed:abstractText |
Here we describe the isolation and nucleotide sequence of a human glycophorin B cDNA. The cDNA was identified by differential hybridization of synthetic oligonucleotide probes to a human erythroleukemic cell line (K562) cDNA library constructed in phage vector lambda gt10. The nucleotide sequence of the glycophorin B cDNA was compared with that of a previously cloned glycophorin A cDNA. The nucleotide sequences encoding the NH2-terminal leader peptide and first 26 amino acids of the two proteins are nearly identical. This homologous region is followed by areas specific to either glycophorin A or B and a number of small regions of homology, which in turn are followed by a very homologous region encoding the presumed membrane-spanning portion of the proteins. We used RNA blot hybridization with both cDNA and synthetic oligonucleotide probes to prove our previous hypothesis that glycophorin B is encoded by a single 0.5- to 0.6-kb mRNA and to show that glycophorins A and B are negatively and coordinately regulated by a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate. We established the intron/exon structure of the glycophorin A and B genes by oligonucleotide mapping; the results suggest a complex evolution of the glycophorin genes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-163658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-2416746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-276884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-3456608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-3571235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-3755722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-3855301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-6198242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-622188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-6259827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-6287227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-6329026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-6489095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-7106126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-728384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-7306980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-732312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3477806-911786
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6735-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3477806-Amino Acid Sequence,
pubmed-meshheading:3477806-Base Sequence,
pubmed-meshheading:3477806-Cell Line,
pubmed-meshheading:3477806-Cloning, Molecular,
pubmed-meshheading:3477806-DNA,
pubmed-meshheading:3477806-Genes,
pubmed-meshheading:3477806-Glycophorin,
pubmed-meshheading:3477806-Humans,
pubmed-meshheading:3477806-Leukemia, Myeloid,
pubmed-meshheading:3477806-Leukemia, Myeloid, Acute,
pubmed-meshheading:3477806-Molecular Sequence Data,
pubmed-meshheading:3477806-Nucleic Acid Hybridization,
pubmed-meshheading:3477806-Sialoglycoproteins
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pubmed:year |
1987
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pubmed:articleTitle |
Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A.
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pubmed:affiliation |
La Jolla Cancer Research Foundation, Cancer Research Center, La Jolla, CA 92037.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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