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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1978-6-12
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pubmed:abstractText |
Purified preparations of the tRNA methylase deficient in supK strains of Salmonella typhimurium transfer methyl groups from S-adenosylmethionine (SAM) to at least two tRNA species, an alanine tRNA and a serine tRNA. The identity of the tRNA substrates for this enzyme was determined by a change in the elution position of the methyl-labeled tRNA from BND-cellulose columns before and after aminoacylation with a specific amino acid followed by derivatization of the free primary amino group with phenoxy- or naphthoxyacetate. The radioactive methyl group enzymatically added to these tRNAs is both acid and base labile and can be hydrolyzed to a volatile product at pHs above 7.5 and also at pH 1. The methylated 3'-nucleotide isolated from digested tRNA is a pyrimidine derivative and chromatographs like a modified uridylic acid. Its identity has not been established, but it is likely that it corresponds to the methyl ester of V, uridin-5-oxyacetic acid.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1041-57
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pubmed:dateRevised |
2010-9-2
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pubmed:meshHeading |
pubmed-meshheading:347399-Alanine,
pubmed-meshheading:347399-Anticodon,
pubmed-meshheading:347399-Hydrolysis,
pubmed-meshheading:347399-RNA, Bacterial,
pubmed-meshheading:347399-RNA, Transfer,
pubmed-meshheading:347399-Salmonella typhimurium,
pubmed-meshheading:347399-Serine,
pubmed-meshheading:347399-Uridine,
pubmed-meshheading:347399-tRNA Methyltransferases
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pubmed:year |
1978
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pubmed:articleTitle |
The identification of the tRNA substrates for the supK tRNA methylase.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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