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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1986-11-17
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pubmed:abstractText |
We have studied the effects of activators of the Ca++- and phospholipid-dependent enzyme protein kinase C on isometric tension development by both intact and skinned coronary artery strips. The intact strips contracted upon incubation with 12-O-tetradecanoylphorbol-13-acetate. 12-O-tetradecanoylphorbol-13-acetate produced a leftward shift in the concentration-response relationship for contraction of the tissues by K+, histamine and norepinephrine. Phorbol-12,13-dibutyrate elicited contraction of detergent-skinned artery strips when the free Ca++ concentration in the bathing media was 0.1 microM or greater. This effect was diminished greatly in the presence of polymyxin B, a putative inhibitor of protein kinase C. Phorbol-12,13-dibutyrate shifted the Ca++ concentration-tension response relationship for the skinned tissue to the left. These results are consistent with a role for protein kinase C in regulating the contractile responses of coronary arterial smooth muscle to a variety of stimuli, at least in part by increasing the sensitivity of the contractile apparatus to Ca++.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3565
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
239
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
38-42
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:3463739-Animals,
pubmed-meshheading:3463739-Calcium,
pubmed-meshheading:3463739-Drug Synergism,
pubmed-meshheading:3463739-Histamine,
pubmed-meshheading:3463739-Muscle, Smooth, Vascular,
pubmed-meshheading:3463739-Muscle Contraction,
pubmed-meshheading:3463739-Norepinephrine,
pubmed-meshheading:3463739-Phorbol 12,13-Dibutyrate,
pubmed-meshheading:3463739-Phorbol Esters,
pubmed-meshheading:3463739-Polymyxin B,
pubmed-meshheading:3463739-Potassium,
pubmed-meshheading:3463739-Protein Kinase C,
pubmed-meshheading:3463739-Swine,
pubmed-meshheading:3463739-Tetradecanoylphorbol Acetate
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pubmed:year |
1986
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pubmed:articleTitle |
Phorbol diesters alter the contractile responses of porcine coronary artery.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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