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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1986-7-9
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pubmed:abstractText |
We report the complete thermodynamic library of all 10 Watson-Crick DNA nearest-neighbor interactions. We obtained the relevant thermodynamic data from calorimetric studies on 19 DNA oligomers and 9 DNA polymers. We show how these thermodynamic data can be used to calculate the stability and predict the temperature-dependent behavior of any DNA duplex structure from knowledge of its base sequence. We illustrate our method of calculation by using the nearest-neighbor data to predict transition enthalpies and free energies for a series of DNA oligomers. These predicted values are in excellent agreement with the corresponding values determined experimentally. This agreement demonstrates that a DNA duplex structure thermodynamically can be considered to be the sum of its nearest-neighbor interactions. Armed with this knowledge and the nearest-neighbor thermodynamic data reported here, scientists now will be able to predict the stability (delta G degree) and the melting behavior (delta H degree) of any DNA duplex structure from inspection of its primary sequence. This capability should prove valuable in numerous applications, such as predicting the stability of a probe-gene complex; selecting optimal conditions for a hybridization experiment; deciding on the minimum length of a probe; predicting the influence of a specific transversion or transition on the stability of an affected DNA region; and predicting the relative stabilities of local domains within a DNA duplex.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-1214299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-14200383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-4022774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-423961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-4370567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-4519026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-4597847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-4680707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-4715013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-4927725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-5237214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-5492018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-6086053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-6159577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-6246822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-6261793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-6401107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-6850063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-7066294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-7066295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-7066296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-7066297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-7171732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-7225341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-7260332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3459152-7326358
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3746-50
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1986
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pubmed:articleTitle |
Predicting DNA duplex stability from the base sequence.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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