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pubmed-article:3443654pubmed:abstractTextA gas chromatographic method with a glass capillary column and electron-capture detection is proposed for the characterization and quantification of 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine (PAF C16) using the corresponding 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-heptafluorobutyrate derivative and, as an internal standard, 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine. The reproducibility was approximately 5% and amounts as low as 20 pg could be measured. The method was specific and allowed the quantification of PAF C16 in supernatants from stimulated human polymorphonuclear neutrophils.lld:pubmed
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pubmed-article:3443654pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:3443654pubmed:articleTitleCharacterization and quantification of PAF-acether (platelet-activating factor) as a heptafluorobutyrate derivative of 1-O-alkyl-2-acetyl-sn-glycerol by capillary column gas chromatography with electron-capture detection.lld:pubmed
pubmed-article:3443654pubmed:affiliationLaboratoire de Chimie Analytique II, S.A.M.M., Faculté de Pharmacie, Chatenay Malabry, France.lld:pubmed
pubmed-article:3443654pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3443654pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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