rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1988-5-2
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pubmed:abstractText |
A gas chromatographic method with a glass capillary column and electron-capture detection is proposed for the characterization and quantification of 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine (PAF C16) using the corresponding 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-heptafluorobutyrate derivative and, as an internal standard, 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine. The reproducibility was approximately 5% and amounts as low as 20 pg could be measured. The method was specific and allowed the quantification of PAF C16 in supernatants from stimulated human polymorphonuclear neutrophils.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
|
pubmed:issn |
0021-9673
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
423
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3443654-Chromatography, Gas,
pubmed-meshheading:3443654-Electrochemistry,
pubmed-meshheading:3443654-Fluorocarbons,
pubmed-meshheading:3443654-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:3443654-Humans,
pubmed-meshheading:3443654-Hydrolysis,
pubmed-meshheading:3443654-Indicators and Reagents,
pubmed-meshheading:3443654-Neutrophils,
pubmed-meshheading:3443654-Platelet Activating Factor,
pubmed-meshheading:3443654-Subcellular Fractions,
pubmed-meshheading:3443654-Triglycerides
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pubmed:year |
1987
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pubmed:articleTitle |
Characterization and quantification of PAF-acether (platelet-activating factor) as a heptafluorobutyrate derivative of 1-O-alkyl-2-acetyl-sn-glycerol by capillary column gas chromatography with electron-capture detection.
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pubmed:affiliation |
Laboratoire de Chimie Analytique II, S.A.M.M., Faculté de Pharmacie, Chatenay Malabry, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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