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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1988-3-4
|
| pubmed:abstractText |
An easy, rapid, and sensitive assay that permits measurements of androgen-binding protein (ABP) in tissue as well as in spent media from Sertoli cells is described; this method involves the specific binding of labeled dihydrotestosterone (DHT) to ABP. The apparatus holds 36 minicolumns loaded with a DEAE Bio-Gel matrix. A peristaltic pump is used for the free fraction elution, taking into account the extremely rapid rate of dissociation of the ABP-DHT complexes. This technique, which allows Scatchard plot analysis, has been used to measure the rates of association (5.15 X 10(5)M-1 S-1 and dissociation (21.32 X 10(-4) S-1; t 1/2 = 5.5 min): the ratio of these rate constants is in perfect agreement with equilibrium dissociation constants determined by Scatchard plot analysis (KD = 4-4.5 nM). The intraassay and interassay coefficients of variation are 5 and 8%, respectively. A good correlation (r = 0.98) is obtained with the standard method of steady-state polyacrylamide gel electrophoresis below a value of 250 micrograms cytosolic proteins/gel. This apparatus, which allows either the measurement of ABP in 12 samples (in triplicates) at a saturating concentration or the analysis of two Scatchard plots (each of 6 points), is also very useful for a rapid localization of ABP during chromatographic purification.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
167
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
167-73
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3434794-Androgen-Binding Protein,
pubmed-meshheading:3434794-Animals,
pubmed-meshheading:3434794-Chromatography, Ion Exchange,
pubmed-meshheading:3434794-Dihydrotestosterone,
pubmed-meshheading:3434794-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3434794-Male,
pubmed-meshheading:3434794-Rats,
pubmed-meshheading:3434794-Rats, Inbred Strains,
pubmed-meshheading:3434794-Sertoli Cells
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
A minicolumn apparatus for androgen-binding protein measurement.
|
| pubmed:affiliation |
Laboratoire de Biochimie, GS CNRS 79, CHU Côte de Nacre, Caen, France.
|
| pubmed:publicationType |
Journal Article
|