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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-1-27
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pubmed:abstractText |
1. Synthetic peptides corresponding to the five, seven, nine and eleven C-terminal amino acids of the tetradecapeptide bombesin as well as bombesin itself and gastrin-releasing peptide have been evaluated in Swiss 3T3 cells in order to define the minimal peptide length needed for biological responsiveness. 2. Gastrin-releasing peptide, bombesin, the undecapeptide and nonapeptide had nearly equipotent abilities to compete for binding of labelled gastrin-releasing peptide to the cell receptors and showed half-maximal competition at 5-10 nM. The heptapeptide and pentapeptide were ineffective. 3. Cross-linking experiments demonstrated specific binding of gastrin-releasing peptide to a 100 kDa receptor subunit. 4. Total cell protein synthesis was stimulated equally by the nonapeptide and longer peptides with a half-maximal effect at 0.5 nM, while a more than 1000-fold higher concentration of the heptapeptide was required to produce a similar response. Comparable results were found when insulin was also present. 5. Neither an inhibition of protein breakdown nor a stimulation of DNA labelling could be demonstrated by bombesin or gastrin-releasing peptide. 6. We conclude that a C-terminal peptide ligand comprising more than seven but no more than nine amino acids is required to achieve high-affinity binding and receptor-mediated responses via the bombesin receptor.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-1137083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-1201380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-184111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-216015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-2423536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-2423543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-2431903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-2999772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-3079915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-3465038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-3493317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-361511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-3749030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-3758207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-3889514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-3954725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-3954726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-4537042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-6143320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-6159649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-6263874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-6344074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-6546686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-6853532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3426545-6882442
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
247
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
427-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3426545-Amino Acid Sequence,
pubmed-meshheading:3426545-Binding Sites,
pubmed-meshheading:3426545-Bombesin,
pubmed-meshheading:3426545-Cell Line,
pubmed-meshheading:3426545-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3426545-Gastrin-Releasing Peptide,
pubmed-meshheading:3426545-Growth Substances,
pubmed-meshheading:3426545-Ligands,
pubmed-meshheading:3426545-Peptides,
pubmed-meshheading:3426545-Protein Binding,
pubmed-meshheading:3426545-Protein Biosynthesis,
pubmed-meshheading:3426545-Thymidine
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pubmed:year |
1987
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pubmed:articleTitle |
C-terminal bombesin sequence requirements for binding and effects on protein synthesis in Swiss 3T3 cells.
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pubmed:affiliation |
Department of Biochemistry, University of Adelaide, South Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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