Linked Life Data

3399622

Source:http://linkedlifedata.com/resource/pubmed/id/3399622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-9-6
pubmed:abstractText
The uptake of sodium into vesicles isolated from the microvillous, maternal-facing plasma membrane of human placenta was studied. In equilibrium exchange conditions, sodium entry increased with time towards an equilibrium value after 30-60 min. Over 90% of the uptake was into an osmotically active space and the initial rate of uptake was halved by amiloride but unaffected by loop diuretics and capnophorin inhibitors. The apparent inhibition constant (Ki) for amiloride was 3.5 x 10(-6) M and the Michaelis constant (Km) with respect to sodium of the amiloride-sensitive component was 7-11 mM. With an imposed outward H+ gradient, sodium was transiently accumulated within the vesicles. The overshoot was abolished by amiloride and shown, by experiments with FCCP (carbonyl cyanide p-trifluoromethoxyphenylhydrazone) and potassium and valonomycin, not to be dependent on any electrical potential generated by the H+ gradient. The evidence for Na+-H+ exchange at this surface and its possible functions are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0144-8757
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
399-411
pubmed:dateRevised
2010-8-25
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Sodium entry into human placental microvillous (maternal) plasma membrane vesicles.
pubmed:affiliation
Department of Physiology, University, Dundee.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't