|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
14A
|
|
pubmed:dateCreated |
1988-9-8
|
|
pubmed:abstractText |
Endonuclease activity identified in crude preparations of rat and human heart mitochondria has each been partially purified and characterized. Both the rat and human activities purify as a single enzyme that closely resembles the endonuclease of bovine-heart mitochondria (Cummings, O.W. et. al. (1987) J. Biol. Chem. 262:2005-2015). All three enzymes, for example elute similarly during gel filtration and DNA-cellulose chromatography, and exhibit similar enzymatic properties. Although the nucleotide sequences of the mtDNAs indicate that there has occurred an unusual degree of divergence in the displacement-loop region during mammalian evolution, the nucleotide specificities of the mt endonucleases appear highly conserved and show a striking preference for an evolutionarily-conserved sequence tract that is located upstream from the heavy (H)-strand origin of DNA replication (OriH).
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-109836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-1104879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-13797961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-2824480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-2982153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-2996785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-3032959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-3560225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-3571254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-3593731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-3818585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-4212385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-4287861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-4368324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-5723464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-5911529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-6178513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-6300036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-6301949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-7033219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-7120390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-7301592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3399407-942051
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0305-1048
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
25
|
|
pubmed:volume |
16
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
6427-45
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3399407-Animals,
pubmed-meshheading:3399407-Base Sequence,
pubmed-meshheading:3399407-Biological Evolution,
pubmed-meshheading:3399407-Chromatography, Affinity,
pubmed-meshheading:3399407-Chromatography, Gel,
pubmed-meshheading:3399407-DNA, Mitochondrial,
pubmed-meshheading:3399407-DNA, Single-Stranded,
pubmed-meshheading:3399407-Endodeoxyribonucleases,
pubmed-meshheading:3399407-Genes,
pubmed-meshheading:3399407-Humans,
pubmed-meshheading:3399407-Mitochondria, Heart,
pubmed-meshheading:3399407-Rats,
pubmed-meshheading:3399407-Structure-Activity Relationship,
pubmed-meshheading:3399407-Substrate Specificity
|
|
pubmed:year |
1988
|
|
pubmed:articleTitle |
The preference of the mitochondrial endonuclease for a conserved sequence block in mitochondrial DNA is highly conserved during mammalian evolution.
|
|
pubmed:affiliation |
Department of Pathology, Washington University School of Medicine, St Louis, MO 63110.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
