Linked Life Data

3371662

Source:http://linkedlifedata.com/resource/pubmed/id/3371662

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-6-24
pubmed:databankReference
pubmed:abstractText
The pelA gene from Erwinia carotovora strain EC encodes pectate lyase A (PLa). The gene was cloned and sequenced. The PLa was purified from Escherichia coli containing the pelA gene on a recombinant plasmid. The optimum pH for the enzyme reaction is at pH 8.5; the optimum temperature for the enzyme reaction is at 40 degrees C; and the purified PLa alone can macerate potato slices. Nucleotide sequence of pelA and the deduced polypeptide sequence of PLa were compared with the pelB gene of E. carotovora and the deduced amino acid sequence of PLb. The nucleotide sequences are 82% homologous and the amino acid sequences are 88% homologous.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Characterization of the Erwinia carotovora pelA gene and its product pectate lyase A.
pubmed:affiliation
Department of Microbiology, University of California, Los Angeles 90024.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't