Linked Life Data

3371075

Source:http://linkedlifedata.com/resource/pubmed/id/3371075

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-7-14
pubmed:abstractText
A modified procedure for measuring human lymphocyte responses to retinal antigens was introduced and found to be highly sensitive. The main modifications are the use of round bottom culture wells, high antigen concentrations (up to 100 micrograms/ml) and extended incubation time (up to 9 days). Using this procedure, the majority of patients with uveitis, as well as a large proportion of healthy donors were found to respond positively (S.I. greater than or equal to 2.0) toward S-antigen and interphoto-receptor retinoid-binding protein. The responses to the retinal antigens were further enhanced by enriching the cultured lymphocytes for the helper/inducer subset. The responses to the retinal antigens were inferior to those against tuberculin (PPD), in particular in subjects who had been inoculated with BCG. The "secondary" responses to PPD were always higher in magnitude, were stimulated by markedly lower concentrations of antigen and were detected earlier in culture. The notion that the responses to retinal antigen in healthy donors are "primary" in nature was further supported by the findings that (a) lymphocytes from cord blood samples resembled adult lymphocytes in their response to S-Ag and (b) healthy donor lymphocytes which were prestimulated in vitro with S-Ag reacted to this protein by a specific "secondary" fashion. The possible involvement of lymphocytes with reactivity toward retinal antigens in pathogenic autoimmune processes in the eye is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0271-3683
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-402
pubmed:dateRevised
2007-7-27
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Lymphocyte responses to retinal-specific antigens in uveitis patients and healthy subjects.
pubmed:affiliation
Laboratory of Immunology, National Eye Institute, Bethesda, MD 20892.
pubmed:publicationType
Journal Article