Linked Life Data

3370221

Source:http://linkedlifedata.com/resource/pubmed/id/3370221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-6-27
pubmed:abstractText
Elevated osmolarity is known to inhibit secretion from a wide range of cells including bovine adrenal chromaffin cells. The mechanism of this inhibition is unclear but the elevated osmolarity has been proposed to oppose an osmotic driving force involved in exocytotic fusion. Using the fluorescent indicators quene 1 and fura2, we monitored the effect of elevated osmolarity on cytoplasmic pH (pHi) and cytoplasmic free Ca2+ [( Ca2+]i). Elevated osmolarity increased both pHi and [Ca2+]i, but had no effect on the [Ca2+]i rise elicited by either K+ or nicotine. Elevating pHi with NH4Cl was shown to inhibit secretion from chromaffin cells. The elevation of pHi by hyperosmolar solutions is proposed as one of the mechanisms by which elevated osmolarity inhibits secretion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
969
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
The role of cytoplasmic pH in the inhibitory action of high osmolarity on secretion from bovine adrenal chromaffin cells.
pubmed:affiliation
MRC Secretory Control Research Group, University of Liverpool, U.K.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't