Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1988-6-1
pubmed:abstractText
We have examined the binding of luzopeptin, an antitumor antibiotic, to five DNA fragments of varying base composition. The drug forms a tight, possibly covalent, complex with the DNA causing a reduction in mobility on nondenaturing polyacrylamide gels and some smearing of the bands consistent with intramolecular cross-linking of DNA duplexes. DNAase I and micrococcal nuclease footprinting experiments suggest that the drug binds best to regions containing alternating A and T residues, although no consensus di- or trinucleotide sequence emerges. Binding to other sites is not excluded and at moderate ligand concentrations the DNA is almost totally protected from enzyme attack. Ligand-induced enhancement of DNAase I cleavage is observed at both AT and GC-rich regions. The sequence selectivity and characteristics of luzopeptin binding are quite different from those of echinomycin, a bifunctional intercalator of related structure.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-3008083, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-3019386, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-3593730, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-4000957, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-6089341, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-6180762, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-6204275, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-6248230, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-6303566, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-6393053, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-7451357, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-7459330, http://linkedlifedata.com/resource/pubmed/commentcorrection/3362673-985413
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2489-507
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Sequence-specific binding of luzopeptin to DNA.
pubmed:affiliation
Department of Pharmacology, University of Cambridge, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't