Linked Life Data

3361235

Source:http://linkedlifedata.com/resource/pubmed/id/3361235

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1988-6-7
pubmed:abstractText
We describe the distortion caused by sera from patients with coccidioidomycosis in an enzyme-linked immunosorbent assay (ELISA) for coccidioidal antigen. In experimental mixtures without exogenous antigen, immune sera produced an antigen-like effect. In contrast, in mixtures with a relatively large concentration of exogenous antigen, immune sera obscured antigen activity. In both circumstances, preparing specimens with an acidification-heat extraction procedure before assay rectified serum interference, and only in specimens with no exogenous antigen was the correction incomplete. Other evidence, including direct measurement of anticoccidioidal antibody after extraction, suggested that the residual antigen-like activity was not from persistent antibody. Incorporating the extraction procedure into the ELISA of clinical specimens, we found antigen activity in nine of 15 specimens. We conclude that acidification-heat extraction of sera improves detection of coccidioidal antigens and that coccidioidal antigens are present in some patients with coccidioidal infection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-2143
pubmed:author
pubmed:issnType
Print
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
560-5
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Enzyme-linked immunosorbent assay for antigens of Coccidioides immitis: human sera interference corrected by acidification-heat extraction.
pubmed:affiliation
Medical Service, VA Medical Center, Tucson, AZ 85723.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't