Linked Life Data

3355827

Source:http://linkedlifedata.com/resource/pubmed/id/3355827

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-5-26
pubmed:abstractText
A novel method for quantitating secretion is described based on measurements of the cellular uptake of the fluorescent aminoacridine dye quinacrine into low-pH secretory granules. The quinacrine fluorescence remaining in the medium was found to decrease after incubation with increasing numbers of the 2H3 rat basophilic leukemia line. This depletion of dye from the medium decreased after a secretory stimulus. Assuming that quinacrine partitions according to mass action, a quantitative model was derived to allow calculation of the percent secretion from dye uptake data. A good correlation was obtained when the values for the percent secretion determined by the quinacrine uptake method were compared with secretion measured by release of the granule enzyme beta-glucuronidase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
939
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
459-66
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Quantitation of secretion by rat basophilic leukemia cells by measurements of quinacrine uptake.
pubmed:affiliation
Immunology Branch, National Institutes of Health, Bethesda, MD 20892.
pubmed:publicationType
Journal Article