Linked Life Data

3351417

Source:http://linkedlifedata.com/resource/pubmed/id/3351417

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-5-2
pubmed:abstractText
The binding of bovine GH (bGH) to hepatic membranes obtained from steers on either high (3% dry matter of body weight per day) or low (1% dry matter of body weight per day) planes of nutrition with or without an oestradiol-17 beta implant was studied (n = 5 per group). Binding studies were performed on both crude membrane homogenates and on 100,000 g microsomal membrane fractions; identical results were obtained using both preparations. In all four groups of animals, linear Scatchard plots were obtained, but following pretreatment of the membranes with MgCl2 to remove endogenously bound hormone, curvilinear plots were obtained in the groups on the high plane of nutrition. Analysis of these curves suggested the presence of a high- and low-affinity binding site, the high-affinity site being fully occupied in the absence of MgCl2 pretreatment. The specific binding of bGH in MgCl2-pretreated crude membranes was greater (P less than 0.01) in well-fed steers (14.8 +/- 1.6%) than in poorly fed steers (9.8 +/- 0.9%). Scatchard analysis showed this to be due to the presence of a high-affinity site (dissociation constant (Kd) = 11.6 +/- 3.3 pmol/l) in the well-fed animals only. In addition, there was an increase (P less than 0.01) in the affinity, but not in the capacity, of the low-affinity site (Kd = 106.4 +/- 22.8 pmol/l in well-fed steers and 197.0 +/- 23.8 pmol/l in poorly fed steers).(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-0795
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-77
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
The somatotrophic axis in young steers: influence of nutritional status and oestradiol-17 beta on hepatic high- and low-affinity somatotrophic binding sites.
pubmed:affiliation
Department of Paediatrics, University of Auckland, New Zealand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't