| pubmed-article:3348416 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C0687028 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C0025148 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C0001629 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C0205102 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C1550278 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C1516698 | lld:lifeskim |
| pubmed-article:3348416 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:3348416 | pubmed:issue | 3 Pt 2 | lld:pubmed |
| pubmed-article:3348416 | pubmed:dateCreated | 1988-4-19 | lld:pubmed |
| pubmed-article:3348416 | pubmed:abstractText | Inner medullary collecting duct cells were isolated from rat papillae and grown to confluence on cover slips. H+ secretion was estimated by intracellular pH (pHi) changes measured with the fluorescent probe 2,7-biscarboxyethyl-5(6)-carboxyfluorescein. In buffered NaCl, pHi was 7.14 +/- 0.04 (n = 78). After acidification about 40% of monolayers exhibited Na+-independent alkalinization. In 5 mM glucose, cell alkalinization occurred at a rate of 47 +/- 4 nM H+/min. However, cell alkalinization did not occur in the presence of 2-deoxy-D-glucose (5-15 mM), iodoacetate (5 mM), or KCN (5 mM). All monolayers tested exhibited amiloride-inhibitable Na+-dependent cell alkalinization that appeared to be a first-order kinetic process; Km [Na+] was approximately 52 mM and Vmax was approximately 250 nM [H+]/min. At a constant extracellular [Na+] (110 mM), Na+-dependent H+ efflux was a first-order function of pHi; Km for intracellular [H+] = 321 nM and Vmax = 182 nM H+/min. The data are consistent with the presence of a primary active H+ pump and a secondary active Na+ exchanger. The metabolic energy for the active H+ pump can be provided by glycolysis and oxidative phosphorylation. | lld:pubmed |
| pubmed-article:3348416 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3348416 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3348416 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3348416 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3348416 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:3348416 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:3348416 | pubmed:author | pubmed-author:SchwartzJ HJH | lld:pubmed |
| pubmed-article:3348416 | pubmed:author | pubmed-author:BengeleH HHH | lld:pubmed |
| pubmed-article:3348416 | pubmed:author | pubmed-author:AlexanderE... | lld:pubmed |
| pubmed-article:3348416 | pubmed:author | pubmed-author:SelvaggioA... | lld:pubmed |
| pubmed-article:3348416 | pubmed:author | pubmed-author:GordonF DFD | lld:pubmed |
| pubmed-article:3348416 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3348416 | pubmed:volume | 254 | lld:pubmed |
| pubmed-article:3348416 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3348416 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3348416 | pubmed:pagination | F391-400 | lld:pubmed |
| pubmed-article:3348416 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:meshHeading | pubmed-meshheading:3348416-... | lld:pubmed |
| pubmed-article:3348416 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3348416 | pubmed:articleTitle | Mechanisms of H+ secretion by inner medullary collecting duct cells. | lld:pubmed |
| pubmed-article:3348416 | pubmed:affiliation | Thorndike Memorial Laboratory, Boston City Hospital, Massachusetts. | lld:pubmed |
| pubmed-article:3348416 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3348416 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:3348416 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3348416 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3348416 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3348416 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3348416 | lld:pubmed |
