Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1988-5-4
pubmed:abstractText
We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We have constructed gene fusions using the cauliflower mosaic virus (CaMV) 35S promoter or the promoter from a gene encoding the small subunit of ribulose bisphosphate carboxylase (rbcS) to direct the expression of beta-glucuronidase in transformed plants. Expression of GUS can be measured accurately using fluorometric assays of very small amounts of transformed plant tissue. Plants expressing GUS are normal, healthy and fertile. GUS is very stable, and tissue extracts continue to show high levels of GUS activity after prolonged storage. Histochemical analysis has been used to demonstrate the localization of gene activity in cells and tissues of transformed plants.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-15929216, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-16453464, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-17758108, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-17781445, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-17812887, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-3035911, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-31918, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-3295256, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-3534890, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-3969146, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-3974711, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-4000958, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-4568840, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6095209, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6096215, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6158890, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6191868, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6269464, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6298724, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6308651, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6329026, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-685179, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-6960240, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-7139714, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-7153687, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-7153689, http://linkedlifedata.com/resource/pubmed/commentcorrection/3327686-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3901-7
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
pubmed:affiliation
Department of Molecular Genetics, Plant Breeding Institute, Trumpington, Cambridge, UK.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't