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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1988-3-15
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pubmed:abstractText |
In the course of acute rejection, myocardial tissue undergoes massive transformation and we hypothetized that for digitalis-like substances, receptor binding characteristics might be altered. Ten canine heterotopic cardiac allografts were carried out and were harvested once rejection had developed (8-10 days post-transplant). Microsomal membrane fractions of those grafts and of native hearts were isolated. Radioligand binding studies were carried out in a medium containing 5 mM Tris PO4, 50 mM Tris HCl, 5 mM MgCl2, pH 7.4 at 37 degrees C, using 3H-ouabain as the ligand. Saturation experiments (n = 10) indicate the presence of one homogeneous population of high affinity binding sites with an affinity constant (Kd) of 8-13 nM and a maximum binding capacity (Bmax) of 47 +/- 3.5 pmol/mg protein. Both saturation and competition binding studies illustrate the fact that acute rejection resulted in a significant decrease in Bmax (43%) without significant alteration in Kd value. These studies indicate that digitalis-like substances might not exert significant inotropic activity during rejection, but this hypothesis must be confirmed by in vivo haemodynamic experiments.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Digitalis Glycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Drug,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase,
http://linkedlifedata.com/resource/pubmed/chemical/digitalis receptor
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pubmed:status |
MEDLINE
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pubmed:issn |
0300-8428
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
537-43
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:3325029-Animals,
pubmed-meshheading:3325029-Binding Sites,
pubmed-meshheading:3325029-Digitalis Glycosides,
pubmed-meshheading:3325029-Dogs,
pubmed-meshheading:3325029-Graft Rejection,
pubmed-meshheading:3325029-Heart Transplantation,
pubmed-meshheading:3325029-Ouabain,
pubmed-meshheading:3325029-Radioligand Assay,
pubmed-meshheading:3325029-Receptors, Drug,
pubmed-meshheading:3325029-Sodium-Potassium-Exchanging ATPase
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pubmed:articleTitle |
Decreased digitalis receptor activity in acute rejecting canine transplanted heart.
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pubmed:affiliation |
Départment de pharmacologie, Faculté de Médecine, Université de Montréal, Québec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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