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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1979-4-25
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pubmed:abstractText |
Serial culture of Rhodospirillum rubrum with 2% CO2 in H2 as the exclusive carbon source resulted in a rather large fraction of the soluble protein (greater than 40%) being comprised of ribulosebisphosphate carboxylase (about sixfold higher than the highest value previously reported). Isolation of the enzyme from these cells revealed that it has physical and kinetic properties similar to those previously described for the enzyme derived from cells grown on butyrate. Notably, the small subunit (which is a constituent of the carboxylase from eucaryotes and most procaryotes) was absent in the enzyme from autotrophically grown R. rubrum. Edman degradation of the purified enzyme revealed that the NH2 terminus is free (in contrast to the catalytic subunit of the carboxylase from eucaryotes) and that the NH2-terminal sequence is Met-Asp-Gln-Ser-Ser-Arg-Tyr-Val-Asn-Leu-Ala-Leu-Lys-Glu-Glu-Asp-Leu-Ile-Ala-Gly-Gly-Glx-His-Val-Leu-. Crystals of the enzyme were readily obtained by dialysis against distilled water.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/33152-1094907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/33152-1152035,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/33152-16592389,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/33152-670222,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/33152-848758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/33152-970962
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
137
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
490-501
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pubmed:dateRevised |
2010-8-31
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pubmed:meshHeading |
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