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rdf:type |
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lifeskim:mentions |
umls-concept:C0011306,
umls-concept:C0032659,
umls-concept:C0039194,
umls-concept:C0086418,
umls-concept:C0334094,
umls-concept:C0457784,
umls-concept:C0871261,
umls-concept:C1291775,
umls-concept:C1521721,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
3
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pubmed:dateCreated |
1987-12-14
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pubmed:abstractText |
The efficacy of dendritic cells in antigen presentation was studied in eight healthy subjects using a lymphoproliferation assay. Both particulate (Mycobacterium leprae, H37Ra) and soluble (PPD, tetanus toxoid) bacterial antigens were used as stimulants over a concentration range of accessory cells (monocytes (MO) and dendritic cells (DC)) varying from 10 to 0.1% in co-cultures using T-enriched cells. In general, co-cultures with T + MO and T + DC at all concentrations of accessory cells showed significant improvement of antigen-induced lymphoproliferation over PBMC cultures. The improvement in delta ct/min of T + DC co-cultures as compared to T + MO with parallel concentrations of accessory cells (P less than 0.05 to less than 0.01) was significant. Of the bacterial antigens used to test the antigen-presenting ability of DC, the particulate antigen (H37Ra) showed the most impressive improvement (380%) of T cell proliferation in DC reconstituted cultures as compared to monocytes. PPD, soluble protein derived from a similar tuberculosis strain of mycobacteria, was not presented as effectively as the particulate equivalent even though the donors of the appropriate cell combinations showed skin test reactivity to this antigen.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-3918113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-4573839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-5549896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6190974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6215197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6391762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6456222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6460832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6605410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6605932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6948298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-6968911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-7021836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311498-762493
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
611-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3311498-Antigens, Bacterial,
pubmed-meshheading:3311498-Cell Division,
pubmed-meshheading:3311498-Dendritic Cells,
pubmed-meshheading:3311498-Female,
pubmed-meshheading:3311498-Humans,
pubmed-meshheading:3311498-Lymphocyte Activation,
pubmed-meshheading:3311498-Male,
pubmed-meshheading:3311498-Monocytes,
pubmed-meshheading:3311498-Mycobacterium,
pubmed-meshheading:3311498-Mycobacterium leprae,
pubmed-meshheading:3311498-T-Lymphocytes,
pubmed-meshheading:3311498-Tetanus Toxoid,
pubmed-meshheading:3311498-Tuberculin
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pubmed:year |
1987
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pubmed:articleTitle |
Human T cell proliferative responses to particulate microbial antigens are supported by populations enriched in dendritic cells.
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pubmed:affiliation |
Department of Pathology, All India Institute of Medical Sciences, New Delhi.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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