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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1987-8-12
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pubmed:abstractText |
The Ca2+-binding proteins parvalbumin (Mr = 12K) and calbindin D28K [previously designated vitamin D-dependent Ca2+-binding protein (Mr = 28K)] are neuronal markers, but their functional roles in mammalian brain are unknown. The expression of these two proteins was studied by immunocytochemical methods in serum-free cultures of hypothalamic cells from 16-day-old fetal mice. Parvalbumin is first detected in all immature neurons, but during differentiation, the number of parvalbumin-immunoreactive neurons greatly declines to a level reminiscent of that observed in vivo, where only a subpopulation of neurons stains for parvalbumin. In contrast, calbindin D28K was expressed throughout the period investigated only in a distinct subpopulation of neurons. Depolarization of fully differentiated hypothalamic neurons in culture resulted in a dramatic decrease of parvalbumin immunoreactivity but not of calbindin D28K immunoreactivity. The parvalbumin staining was restored on repolarization. Because the anti-parvalbumin serum seems to recognize only the metal-bound form of parvalbumin, the loss of immunoreactivity may signal a release of Ca2+ from intracellular parvalbumin during depolarization of the cells. We suggest that parvalbumin might be involved in Ca2+-dependent processes associated with neurotransmitter release.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-3042
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
442-51
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3298546-Animals,
pubmed-meshheading:3298546-Calcium-Binding Protein, Vitamin D-Dependent,
pubmed-meshheading:3298546-Cell Division,
pubmed-meshheading:3298546-Cells, Cultured,
pubmed-meshheading:3298546-Fetus,
pubmed-meshheading:3298546-Fluorescent Antibody Technique,
pubmed-meshheading:3298546-Hypothalamus,
pubmed-meshheading:3298546-Mice,
pubmed-meshheading:3298546-Molecular Weight,
pubmed-meshheading:3298546-Muscle Proteins,
pubmed-meshheading:3298546-Neurons,
pubmed-meshheading:3298546-Parvalbumins
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pubmed:year |
1987
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pubmed:articleTitle |
Developmental and functional studies of parvalbumin and calbindin D28K in hypothalamic neurons grown in serum-free medium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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