Linked Life Data

3296650

Source:http://linkedlifedata.com/resource/pubmed/id/3296650

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-7-10
pubmed:abstractText
A colonization factor antigen (CFA)-deficient mutant has been isolated that is as efficient as the CFA/I-carrying, enterotoxin-producing E. coli strain it was derived from in producing diarrhoea and colonizing the intestine in a rabbit non-ligated intestine model, the RITARD model. Infection with 10(11) mutant bacteria induced diarrhoea in 15 of the 17 rabbits challenged; corresponding diarrhoeal attack rates after challenge with similar doses of the original CFA/I-positive strain and of a non-enterotoxinogenic, non-CFA control strain were 23/24 and 0/15, respectively. Whereas the mean time of excreting the challenge strain in faeces did not differ between the mutant and the CFA/I-positive strain (4.5 and 4.3 days, respectively), it was significantly longer (p less than 0.05) than for the control strain (2.5 days). Similarly, the magnitude of the serum antibody responses to homologous O-antigen was equally high after infection with the mutant and the CFA/I-positive strain and considerably higher than after challenge with the negative control strain. An initial infection in the RITARD model with the CFA-deficient mutant offered highly significant protection against diarrhoea (p less than 0.001) as well as colonization (p less than 0.01) on subsequent challenge with the original CFA/I-positive strain. No significant protection, either against colonization or disease, was induced by initial infection with a similar dose of the control strain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0108-0180
pubmed:author
pubmed:issnType
Print
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-30
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Colonization, diarrhoea and protective immunogenicity of a CFA-deficient, enterotoxin-producing Escherichia coli mutant in a non-ligated intestine experimental model.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't