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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1988-7-8
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pubmed:abstractText |
A technique to quantify tissue fibronectin was developed, using peroxidase-antiperoxidase immunocytochemistry and automated scanning light microscopy. This technique was developed using isolated perfused rat lungs, some of which were subjected to acute oxidant lung injury. Both injured and control lungs were perfused with solutions containing heterologous fibronectin. The technique clearly demonstrated differences in the amount of tissue fibronectin in injured and noninjured lung as well as differences between lungs exposed to fibronectin and those not exposed. The described technique offers a reliable method for quantifying tissue fibronectin and is sensitive enough to detect differences in tissue fibronectin under experimental conditions of acute lung injury.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0037-9727
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
188
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-68
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pubmed:dateRevised |
2007-11-2
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pubmed:meshHeading |
pubmed-meshheading:3287385-Animals,
pubmed-meshheading:3287385-Fibronectins,
pubmed-meshheading:3287385-Glucose Oxidase,
pubmed-meshheading:3287385-Histocytochemistry,
pubmed-meshheading:3287385-Immunoenzyme Techniques,
pubmed-meshheading:3287385-Lung,
pubmed-meshheading:3287385-Lung Diseases,
pubmed-meshheading:3287385-Male,
pubmed-meshheading:3287385-Rats,
pubmed-meshheading:3287385-Rats, Inbred Strains
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pubmed:year |
1988
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pubmed:articleTitle |
An immunocytochemical method for quantification of lung tissue fibronectin.
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pubmed:affiliation |
Department of Veterinary Science, University of Wisconsin, Madison 53706.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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