Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
|
pubmed:dateCreated |
1988-6-9
|
pubmed:abstractText |
A novel neutrophil-activating peptide is detected in supernatants from mitogen-stimulated human T lymphocyte preparations. This chemotaxin was purified to apparent homogeneity by sequential Gel G-75 permeation chromatography, wide pore reversed phase (RP-8) HPLC, size exclusion HPLC, and reversed phase (RP-18) HPLC. Additional characterization of this lymphocyte-derived neutrophil-activating peptide (LYNAP) resulted in a single peak upon reversed phase HPLC and size exclusion HPLC. SDS-PAGE under nonreducing conditions revealed a single line at 10 kDa. LYNAP stimulated neutrophil chemotaxis (ED50 of 3 +/- 3 ng/ml), chemokinesis (ED50 of 2 +/- 2 ng/ml), and caused degranulation of cytochalasin B pretreated human neutrophils (ED50 of 20 ng/ml). In purified human monocytes, chemotactic responses to LYNAP at doses up to 100 ng/ml were absent, indicating nonidentity with a lymphocyte-derived monocyte chemotactic factor previously described by other workers. LYNAP shows biochemical and biologic similarities to a recently detected monocyte-derived neutrophil-activating peptide (MONAP). Moreover, desensitization experiments revealed cross-deactivation between LYNAP and MONAP, not, however, between these two chemotactic peptides and other well characterized polymorphonuclear leukocyte chemotaxins, e.g., C5a, FMLP, leukotriene B4, or platelet-activating factor. This finding points toward structure identity or homology of both chemotaxins, MONAP and LYNAP.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0022-1767
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
140
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3534-40
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:3283239-Chemistry, Physical,
pubmed-meshheading:3283239-Chemotactic Factors,
pubmed-meshheading:3283239-Chemotaxis, Leukocyte,
pubmed-meshheading:3283239-Cross Reactions,
pubmed-meshheading:3283239-Dose-Response Relationship, Drug,
pubmed-meshheading:3283239-Glucuronidase,
pubmed-meshheading:3283239-Humans,
pubmed-meshheading:3283239-Interleukin-8,
pubmed-meshheading:3283239-Lymphokines,
pubmed-meshheading:3283239-Monocytes,
pubmed-meshheading:3283239-Neutrophils,
pubmed-meshheading:3283239-Physicochemical Phenomena,
pubmed-meshheading:3283239-T-Lymphocytes
|
pubmed:year |
1988
|
pubmed:articleTitle |
Purification and partial biologic characterization of a human lymphocyte-derived peptide with potent neutrophil-stimulating activity.
|
pubmed:affiliation |
Department of Dermatology, University of Kiel, Federal Republic of Germany.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|