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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1988-6-2
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pubmed:abstractText |
We isolated Microtus agrestis-mouse somatic cell hybrid clones which had retained either the active or the inactive M. agrestis X chromosome. In both hybrid clones the X chromosomes retained their original chromatin conformation as studied by the in situ nick translation technique--the active X chromosome retained its high sensitivity to DNase I while the inactive one remained insensitive. A clone in which the hypoxanthine guanine phosphoribosyltransferase (HPRT) gene had been spontaneously reactivated was isolated from the hybrid containing the inactive X chromosome. The in situ nick translation technique was used to study possible DNA conformation changes in the euchromatin of the inactive X chromosome with special reference to the reactivated HPRT locus. We found that the euchromatin in this X chromosome exhibited the same low sensitivity to DNase I as is characteristic of the inactive X chromosome.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0009-5915
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
227-30
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3282832-Animals,
pubmed-meshheading:3282832-Arvicolinae,
pubmed-meshheading:3282832-Chromatin,
pubmed-meshheading:3282832-Deoxyribonuclease I,
pubmed-meshheading:3282832-Dosage Compensation, Genetic,
pubmed-meshheading:3282832-Euchromatin,
pubmed-meshheading:3282832-Female,
pubmed-meshheading:3282832-Hybrid Cells,
pubmed-meshheading:3282832-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:3282832-Mice,
pubmed-meshheading:3282832-X Chromosome
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pubmed:year |
1988
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pubmed:articleTitle |
DNase I sensitivity of Microtus agrestis active, inactive and reactivated X chromosomes in mouse-Microtus cell hybrids.
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pubmed:affiliation |
Department of Genetics, Hebrew University, Jerusalem, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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