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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-3-9
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pubmed:abstractText |
E protein, the 29 Kd repE gene product, is essential for the replication of the Escherichia coli F plasmid. The repE gene has been cloned and expressed in an inducible ATG-fusion vector, and the protein product has been purified to homogeneity. The purified E protein is present as a dimer in solution and binds specifically to both the 19-bp direct repeats (incB) found within the mini-F origin of replication ori2 and the repE operator DNA. Examination of the amino acid sequence of E protein revealed a consensus sequence for DNA binding.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-2986188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-2999077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-3016662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-3462754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-3520615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-3898165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-4915295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-4924006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-6201704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-6236744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-6275366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-6293926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-6294461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-6305917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-6353162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-6750320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-7028572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-7050629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3277161-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
413-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.
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pubmed:affiliation |
Department of Biology, University of California, Los Angeles 90024.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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