Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
|
pubmed:dateCreated |
1976-6-2
|
pubmed:abstractText |
Further evidence for the establishment of the pig fallopian tube (PFT) cell line as a continuous cell line was shown by an increase in the maximum population density as the number of subcultures increased. The optimal pH and temperature-growth ranges appeared to be 7.4-7.8 and 37-41 degrees C respectively, and the population doubling time was 20-25 h under optimal growth conditions. With progressive subculture, the serum requirements dropped from 20 to 2%. A plating efficiency of 2 to 4% was found in all serial subcultures. Colonies were observed in agar suspension culture at the 146th subculture and thereafter. Chromosomal alterations were found in the 100th subculture and thereafter.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0008-4166
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
21
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2094-7
|
pubmed:dateRevised |
2003-11-14
|
pubmed:meshHeading |
pubmed-meshheading:3274-Animals,
pubmed-meshheading:3274-Cell Count,
pubmed-meshheading:3274-Cell Division,
pubmed-meshheading:3274-Cell Line,
pubmed-meshheading:3274-Chromosomes,
pubmed-meshheading:3274-Fallopian Tubes,
pubmed-meshheading:3274-Female,
pubmed-meshheading:3274-Hydrogen-Ion Concentration,
pubmed-meshheading:3274-Swine,
pubmed-meshheading:3274-Temperature
|
pubmed:year |
1975
|
pubmed:articleTitle |
Growth characteristics of a cell line derived from the pig oviduct.
|
pubmed:publicationType |
Journal Article
|