Linked Life Data

3264665

Source:http://linkedlifedata.com/resource/pubmed/id/3264665

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-2-9
pubmed:abstractText
In this study we investigated whether elevated levels of the inflammatory mediator granulocyte elastase in seminal plasma were associated with increased numbers of CD4+ T helper/inducer lymphocytes and monocytes/macrophages in semen, the principal host cells of the human immunodeficiency virus (HIV). Semen samples were obtained from 105 men attending an infertility clinic. CD4+ lymphocytes, monocytes/macrophages and cells expressing the common leukocyte antigen (CD45) were identified by monoclonal antibodies (MAb's) in a biotinstreptavidin immunoperoxidase technique. Granulocyte elastase levels in seminal plasma were determined by an enzyme-linked immunosorbent assay. In 17 men, granulocyte elastase levels were higher than 1000 ng/ml seminal plasma, indicating male genital tract inflammation. Compared to men with low/normal granulocyte elastase levels in semen (less than 250 ng/ml), these men showed significantly higher mean numbers of total leukocytes, CD4+ lymphocytes and monocytes/macrophages in semen (P less than 0.001); median cell numbers for the group with high/inflammatory granulocyte elastase levels were increased 38-fold for total leukocytes (19,800,000 versus 520,625 per ejaculate), 19-fold for monocytes/macrophages (2,594,000 versus 134,565), and 6-fold for CD4+ lymphocytes (82,900 versus 14,100). Because of the increased numbers of potential HIV-host cells in inflammatory semen, male genital tract inflammation may be an important cofactor in the sexual transmission of the human immunodeficiency virus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0303-4569
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
404-10
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Male genital tract inflammation associated with increased numbers of potential human immunodeficiency virus host cells in semen.
pubmed:affiliation
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't