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rdf:type |
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lifeskim:mentions |
umls-concept:C0017687,
umls-concept:C0019409,
umls-concept:C0019932,
umls-concept:C0030012,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0227525,
umls-concept:C0441472,
umls-concept:C0521451,
umls-concept:C0599816,
umls-concept:C1749497
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pubmed:issue |
1
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pubmed:dateCreated |
1989-3-17
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pubmed:abstractText |
Hepatocytes isolated from the periportal or perivenous zones of livers of fed rats were used to study the long-term (14 h) and short-term (2 h) effects of glucagon on gluconeogenesis and ketogenesis. Long-term culture with glucagon (100 nM) resulted in a greater increase (P less than 0.01) in gluconeogenesis in periportal than in perivenous cells (93 +/- 16 versus 30 +/- 14 nmol/h per mg of protein; 72% versus 30% increase), but short-term incubation (2 h) with glucagon resulted in similar stimulation in the two cell populations. Rates of ketogenesis (acetoacetate and D-3-hydroxybutyrate production) were not significantly higher in periportal cells cultured without glucagon, compared with perivenous cells. However, after long-term culture with glucagon, the periportal cells had a significantly higher rate of ketogenesis (from either palmitate or octanoate as substrate), but a lower 3-hydroxybutyrate/acetoacetate production ratio, suggesting a more oxidized mitochondrial NADH/NAD+ redox state despite the higher rate of beta-oxidation. Periportal hepatocytes had a higher activity of carnitine palmitoyltransferase but a lower activity of citrate synthase than did perivenous cells. These findings suggest that: (i) glucagon elicits greater long-term stimulation of gluconeogenesis in periportal than in perivenous hepatocytes maintained in culture; (ii) after culture with glucagon, the rates of ketogenesis and the mitochondrial redox state differ in periportal and perivenous hepatocytes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-13664971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-170133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-177845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-201494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-240844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-2886246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-2992454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-2994630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3021146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3097499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3530857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3552523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3593405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3622915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3813565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3814074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3827816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-3932072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-4291787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-521313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-5667361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-6008008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-6136405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-6825695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-7004997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-7380004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223900-889865
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
197-204
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3223900-Animals,
pubmed-meshheading:3223900-Cells, Cultured,
pubmed-meshheading:3223900-DNA,
pubmed-meshheading:3223900-Dose-Response Relationship, Drug,
pubmed-meshheading:3223900-Glucagon,
pubmed-meshheading:3223900-Gluconeogenesis,
pubmed-meshheading:3223900-Ketone Bodies,
pubmed-meshheading:3223900-Liver,
pubmed-meshheading:3223900-Male,
pubmed-meshheading:3223900-Mitochondria, Liver,
pubmed-meshheading:3223900-Oxidation-Reduction,
pubmed-meshheading:3223900-Proteins,
pubmed-meshheading:3223900-Rats,
pubmed-meshheading:3223900-Rats, Inbred Strains,
pubmed-meshheading:3223900-Tissue Distribution
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pubmed:year |
1988
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pubmed:articleTitle |
Glucagon regulation of gluconeogenesis and ketogenesis in periportal and perivenous rat hepatocytes. Heterogeneity of hormone action and of the mitochondrial redox state.
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pubmed:affiliation |
Department of Medicine, University of Newcastle upon Tyne, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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