Linked Life Data

3217923

Source:http://linkedlifedata.com/resource/pubmed/id/3217923

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-2-28
pubmed:abstractText
A method was developed for isolation of the A alpha-chain from S-carboxamidomethylated fibrinogen. A mixture of the three constituent polypeptide chains of human fibrinogen was applied onto a column of concanavalin A-Sepharose. While the carbohydrate-free A alpha-chain was not delayed on the affinity chromatography column, both glycosylated subunit chains, B beta- and gamma-chain, were adsorbed to the insolubilized lectin and were quantitatively eluted from the column with 0.2 M methyl-alpha-D-mannoside. SDS-electrophoresis on polyacrylamide gel was employed for analysis of chromatographic fractions. Complete recovery of the A alpha-chain was observed. The described procedure is very simple and permits isolation of large amounts of pure A alpha-chain from S-carboxyamidomethylated fibrinogen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0340-6245
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
308-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Isolation of fibrinogen A alpha-chain by affinity chromatography on concanavalin A-sepharose.
pubmed:affiliation
Central Hematology Laboratory, Inselspital, Bern, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't