Linked Life Data

320264

Source:http://linkedlifedata.com/resource/pubmed/id/320264

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1977-4-25
pubmed:abstractText
A radioimmunological method for the quantitation of class-specific antibodies has been developed. The method allows the quantitation of nanogram per ml concentrations of IgG- and IgM-anti-DNP antibodies without any physical or chemicadioimmunoassay of class-specific antibodies (RIACA) against BSA (Viljanen et al., 1975) developed recently by us. DNP was coupled covalently to a cyanogen bromide activated paper disk with the augmentation of lysine molecule. Anti-DNP antibodies were allowed to react with the coupled DNP and then quantitated by their capacity to bind 125I-labeled anti-chicken-mu or anti-chicken-gamma. The inter-assay variation coefficients ranged from 8.1 to 14.7% and the mean standard deviations of duplicate determinations were about 11%. The combination of this method with the exact immunoradiometric quantitation of the total serum IgM and IgG, and with an immunoabsorption technique, makes possible to quantitate class-specific antibodies in weight units.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
111-22
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Radioimmunoassay of class-specific antibodies (RIACA): chicken antibodies to DNP.
pubmed:publicationType
Journal Article