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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1977-4-15
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pubmed:abstractText |
The steady-state levels of a number of aminoacyl-transfer ribonucleic acid synthetases are known to be positively correlated with growth rate in Escherichia coli. To describe the regulation of these enzymes during a nutritional shift-up, use was made of the recent identification of polypeptide chains of several synthetases in whole cell lysates resolved by the O'Farrell two-dimensional gel system. A culture growing in acetate minimal medium was shifted to glucose-rich medium and pulse labeled with [3H]leucine and [3H]isoleucine for 30- or 6-s intervals during the 20 min after the shift. After mixing with a uniformly [35S]sulfate-labeled reference culture, the samples were subjected to two-dimensional gel electrophoresis. The 3H/35S ratio in the resolved synthetase polypeptides provided an accurate estimation of their transient rates of synthesis. Five aminoacyl-transfer ribonucleic acid synthetases (those for argnine, glycine, isoleucine, phenylalanine, and valine) exhibited an increase in formation within 30 to 90 s after the shift-up. The magnitude of the increases corresponded to the final steady-state values and were reached within 2 to 3 min. The addition to rifampin revealed that the increase in the differential rate of valyl-transfer ribonucleic acid synthetase formation was the result of increased messenger ribonucleic acid transcription and not of a relaxation of some translation restriction.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-1097418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-1180514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-177401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-318645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-4565494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-4573848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-4576589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-4592258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-4604283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-5340469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-775303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/320192-781673
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
702-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:320192-Acetates,
pubmed-meshheading:320192-Amino Acyl-tRNA Synthetases,
pubmed-meshheading:320192-Arginine-tRNA Ligase,
pubmed-meshheading:320192-Escherichia coli,
pubmed-meshheading:320192-Glucose,
pubmed-meshheading:320192-Glycine-tRNA Ligase,
pubmed-meshheading:320192-Isoleucine-tRNA Ligase,
pubmed-meshheading:320192-Peptide Biosynthesis,
pubmed-meshheading:320192-Phenylalanine-tRNA Ligase,
pubmed-meshheading:320192-Rifampin,
pubmed-meshheading:320192-Valine-tRNA Ligase
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pubmed:year |
1977
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pubmed:articleTitle |
Transient rates of synthesis of five amionacyl-transfer ribonucleic acid synthetases during a shift-up of Escherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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