Linked Life Data

3182582

Source:http://linkedlifedata.com/resource/pubmed/id/3182582

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-12-21
pubmed:abstractText
The mite allergen extracts (Dermatophagoides pteronyssinus and D. farinae), were fractionated by polyacrylamide gel electrophoresis and autoradiography. The molecular weight of the most apparent proteins of D. pteronyssinus was between 27,000 and 35,000, and the protein components of D. farinae were in the 9-100-kilodalton range. Specific IgE antibodies directed against mite allergens were utilized as probes to detect the allergens. Using immunoblot analysis and radiostained technique, D. pteronyssinus-sensitive patient sera displayed the major protein of allergens with molecular weights of 15,800 and 27,000-31,000, while D. farinae-sensitive patients' sera displayed the major protein of allergens with molecular weights of 12,000, 15,800 and 26,000-29,000. The electroblotting technique is fast, convenient, and highly suitable for both allergen composition studies and screening of antibody specificities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0277-0903
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Detection of house dust mite allergens and immunoblot analysis in asthmatic children.
pubmed:affiliation
Department of Pediatrics, Veterans General Hospital, Taipei, Taiwan, Republic of China.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't