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rdf:type |
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lifeskim:mentions |
umls-concept:C0019704,
umls-concept:C0023978,
umls-concept:C0162493,
umls-concept:C0205245,
umls-concept:C1514562,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
10
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pubmed:dateCreated |
1988-12-22
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pubmed:abstractText |
The transcriptional regulation of the human immunodeficiency virus (HIV) type I involves the interaction of both viral and cellular proteins. The viral protein tat is important in increasing the amount of viral steady-state mRNA and may also play a role in regulating the translational efficiency of viral mRNA. To identify distinct functional domains of tat, oligonucleotide-directed mutagenesis of the tat gene was performed. Point mutations of cysteine residues in three of the four Cys-X-X-Cys sequences in the tat protein resulted in a marked decrease in transcriptional activation of the HIV long terminal repeat. Point mutations which altered the basic C-domain of the protein also resulted in decreases in transcriptional activity, as did a series of mutations that repositioned either the N or C termini of the protein. Conservative mutations of other amino acids in the cysteine-rich or basic regions and in a series of proline residues in the N terminus of the molecule resulted in minimal changes in tat activation. These results suggest that several domains of tat protein are involved in transcriptional activation with the cysteine-rich domain being required for complete activity of the tat protein.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-17748812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2420471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2421409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2825027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2825351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2828663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2867473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2942760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2968158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2981427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2988790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2990040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-2990041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3000623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3002031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3003584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3007995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3012355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3023047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3025848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3028647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3031512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3115591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3119324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3125980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3138113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3257103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3299106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3319186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3428273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3443102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3476953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3490693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3498942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3500398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3530496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3530501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3549279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3550810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3554236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3638988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3643816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3754034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-3935797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-4040853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-6096007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-6189183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-6200936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-6206563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-6218886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-6324345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-6705049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3181132-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3143-7
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:3181132-Nuclear Proteins,
pubmed-meshheading:3181132-Transcription, Genetic,
pubmed-meshheading:3181132-Structure-Activity Relationship,
pubmed-meshheading:3181132-Gene Expression Regulation,
pubmed-meshheading:3181132-Oligodeoxyribonucleotides,
pubmed-meshheading:3181132-DNA-Binding Proteins,
pubmed-meshheading:3181132-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:3181132-Regulatory Sequences, Nucleic Acid
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