Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1988-11-21
pubmed:abstractText
Single-copy sequences can be enzymatically amplified from genomic DNA by the polymerase chain reaction. By using unequal molar amounts of the two amplification primers, it is possible in a single step to amplify a single-copy gene and produce an excess of single-stranded DNA of a chosen strand for direct sequencing or for use as a hybridization probe. Further, individual alleles in a heterozygote can be sequenced directly by using allele-specific oligonucleotides either in the amplification reaction or as sequencing primers. By using these methods, we have studied the allelic diversity at the HLA-DQA locus and its association with the serologically defined HLA-DR and -DQ types. This analysis has revealed a total of eight alleles and three additional haplotypes. This procedure has wide applications in screening for mutations in human genes and facilitates the linking of enzymatic amplification of genes to automated sequencing.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-2448875, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-2842756, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-2881260, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-2890208, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-2999980, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-3296189, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-3340835, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-3368460, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-3431465, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-3461561, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-3683554, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-3785382, http://linkedlifedata.com/resource/pubmed/commentcorrection/3174659-6220406
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7652-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
pubmed:affiliation
Department of Human Genetics, Cetus Corporation, Emeryville, CA 94608.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't