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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1976-4-9
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pubmed:abstractText |
1. 6-Phosphogluconate dehydrogenase from rabbit mammary gland was purified to homogeneity by the criterion of polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. The molecular weight of the subunit is 52 000. The enzyme was purified 150-fold with a final specific activity of 20 mumol of NADP+ reduced/min per mg of protein and overall yield of 3%. The molecular weight of the native enzyme is estimated to be 104 000 from gel-filtration studies. The final purification step was carried out by affinity chromatography with NADP+-Sepharose. 2. The Km values for 6-phosphogluconate and NADP+ are approx. 54 muM and 23 muM respectively. 3. Citrate and pyrophosphate are competitive inhibitors of the enzyme with respect to both 6-phosphogluconate and NADP+. 4. MgCl2 affects the apparent Km for NADP+ at saturating concentrations of 6-phosphogluconate.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-13105646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-14403829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-14487824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-4145695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-4151477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-4286674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-4358619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-4389822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-4401169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-4691505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-4830191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-5025702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-5357014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3163-5862401
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
151
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3163-Ammonium Sulfate,
pubmed-meshheading:3163-Animals,
pubmed-meshheading:3163-Centrifugation,
pubmed-meshheading:3163-Chromatography, Affinity,
pubmed-meshheading:3163-Chromatography, DEAE-Cellulose,
pubmed-meshheading:3163-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3163-Kinetics,
pubmed-meshheading:3163-Magnesium,
pubmed-meshheading:3163-Mammary Glands, Animal,
pubmed-meshheading:3163-Molecular Weight,
pubmed-meshheading:3163-NADP,
pubmed-meshheading:3163-Phosphogluconate Dehydrogenase,
pubmed-meshheading:3163-Proteins,
pubmed-meshheading:3163-Rabbits,
pubmed-meshheading:3163-Sodium Dodecyl Sulfate
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pubmed:year |
1975
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pubmed:articleTitle |
Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland.
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pubmed:publicationType |
Journal Article
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