Linked Life Data

3156381

Source:http://linkedlifedata.com/resource/pubmed/id/3156381

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-4-8
pubmed:abstractText
A sensitive and specific radioimmunoassay for [6-D-tryptophan]luteinizing hormone-releasing hormone [( D-Trp6]LH-RH) was developed and used for following the rate of liberation of [D-Trp6]LH-RH from a long-acting delivery system based on a microcapsule formulation. Rabbit antibodies were generated against [D-Trp6]LH-RH conjugated to bovine serum albumin with glutaraldehyde. Crossreactivity with LH-RH was less than 1%; there was no significant crossreactivity with other peptides. The minimal detectable dose of [D-Trp6]LH-RH was 2 pg per tube. Intra- and interassay coefficients of variation were 8% and 10%, respectively. The radioimmunoassay was suitable for direct determination of [D-Trp6]LH-RH in serum, permitting the study of blood levels of the analog after single injections into normal men and after once-a-month administration of microcapsules to rats. In men, 90 min after subcutaneous injection of 250 micrograms of the peptide, serum [D-Trp6]LH-RH rose to 6-12 ng/ml. Luteinizing hormone was increased 90 min and 24 hr after the administration of the analog. Several batches of microcapsules were tested in rats and the rate of release of [D-Trp6]LH-RH was followed. The improved batch of microcapsules of [D-Trp6]LH-RH increased serum concentrations of the analog for 30 days or longer after intramuscular injection. This was accompanied by suppression of testosterone levels for more than 30 days. This radioimmunoassay should be of value for monitoring [D-Trp6]LH-RH during long-term therapy.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-354980, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-4298696, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6110575, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6117727, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6135908, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6141569, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6146827, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6210706, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6228566, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6237365, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6362868, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6404441, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6461861, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-647914, http://linkedlifedata.com/resource/pubmed/commentcorrection/3156381-6810559
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1547-51
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Radioimmunoassay for 6-D-tryptophan analog of luteinizing hormone-releasing hormone: measurement of serum levels after administration of long-acting microcapsule formulations.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.