Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1985-2-14
|
pubmed:abstractText |
We analyzed the release of activities capable of stimulating the in vitro growth of human hemopoietic progenitor cells by long-term cultured T cell growth factor (TCGF)-dependent human T lymphocytes. Seven cell lines tested produced colony-stimulating activity (CSA) as well as burst-promoting activity (BPA). The CSA stimulated primarily the growth of the cells forming colonies after 14 days of incubation. In addition the supernatants from these seven T-cell lines showed the ability to induce the in vitro growth of mixed granulocyte, erythroid, megakaryocyte, macrophage colonies (CFU-GEMM). The release of hemopoietic factors did not depend on the presence of accessory cells or phytohemagglutinin or serum during the incubation for factor production. In six of the T cell lines the majority of the cells were reactive to the OKT 8 monoclonal antibody (MoAb), whereas one cell line contained mostly OKT 4+ cells. Suppressor activity was detected in three tested OKT 8+ cell lines, while the one OKT 4+ displayed helper activity. All cell lines produced hemopoietic factors with equal efficiency. These results indicate that factors affecting human hematopoiesis are produced by normal T lymphocytes in long-term culture and this property is not related to the helper or suppressor activity of the cultured cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0021-9541
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
122
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
7-13
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:3155522-Adult,
pubmed-meshheading:3155522-Bone Marrow,
pubmed-meshheading:3155522-Cell Line,
pubmed-meshheading:3155522-Colony-Stimulating Factors,
pubmed-meshheading:3155522-Culture Techniques,
pubmed-meshheading:3155522-Humans,
pubmed-meshheading:3155522-Interleukin-2,
pubmed-meshheading:3155522-Phenotype,
pubmed-meshheading:3155522-T-Lymphocytes,
pubmed-meshheading:3155522-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:3155522-T-Lymphocytes, Regulatory
|
pubmed:year |
1985
|
pubmed:articleTitle |
Release of hemopoietic factors by normal human T cell lines with either suppressor or helper activity.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|