Linked Life Data

3132975

Source:http://linkedlifedata.com/resource/pubmed/id/3132975

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-8-3
pubmed:abstractText
To elucidate the effects of acetylcholine on the electrical properties of incompletely confluent Madin Darby canine kidney (MDCK) cells continuous measurements of the potential difference across the cell membrane (PD) were made with conventional microelectrodes during rapid changes of extracellular fluid composition. During control conditions PD averages -48.9 +/- 1.0 mV (n = 51). 1 mumol/l acetylcholine leads to a sustained but reversible hyperpolarization of the cell membrane by -17.9 +/- 0.7 mV (n = 51). Half-maximal effect is observed at some 100 nmol/l. 1 mumol/l atropine does not significantly alter the potential difference across the cell membrane, but abolishes reversibly the hyperpolarizing effect of acetylcholine. Increase of extracellular potassium concentration from 5.4 mmol/l to 20 mmol/l depolarizes the cell membrane by +12.1 +/- 1.1 mV (n = 12) in the absence and by +25.7 +/- 0.9 mV (n = 12) in the presence of acetylcholine. Within 80 s removal of extracellular calcium leads to a depolarization of the cell membrane by +16.2 +/- 3.2 mV (n = 9). In the nominal absence of extracellular calcium acetylcholine leads to a transient hyperpolarization by -13.8 +/- 1.8 mV (n = 9), which can be elicited only once. In conclusion, acetylcholine hyperpolarizes the plasma membrane of MDCK cells by calcium-dependent enhancement of potassium conductance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
941
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Effect of acetylcholine on electrical properties of subconfluent Madin Darby canine kidney cells.
pubmed:affiliation
Institute of Physiology, University of Innsbruck, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't