Linked Life Data

3126151

Source:http://linkedlifedata.com/resource/pubmed/id/3126151

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-4-19
pubmed:abstractText
A clinical isolate (strain 4037) of Kanagawa phenomenon-negative Vibrio parahaemolyticus was studied. Although the strain was judged to be Kanagawa phenomenon-negative by various conventional tests, it produced a new hemolysin (named Vp-TRH, for thermostable direct hemolysin [Vp-TDH]-related hemolysin) that was related to the Vp-TDH produced by ordinary Kanagawa phenomenon-positive V. parahaemolyticus. Vp-TRH was purified by ammonium sulfate fractionation and successive column chromatographies on DEAE-cellulose, hydroxyapatite, and Mono Q. The molecular weight of Vp-TRH was estimated as 48,000 by Sephadex G-100 gel filtration, and the molecular weight of the subunit was estimated to be 23,000 by sodium dodecyl sulfate-slab gel electrophoresis. Thus, like Vp-TDH, Vp-TRH seems to be composed of two subunits. The isoelectric point of Vp-TRH was determined to be 4.6. Vp-TRH showed lytic activities different from those of Vp-TDH on erythrocytes from various animals, especially those from calves, chickens, and sheep. The hemolytic activity of Vp-TRH was labile on heat treatment at 60 degrees C for 10 min, unlike that of Vp-TDH. The immunological similarities, but not the identities of Vp-TRH and Vp-TDH, were demonstrated by Ouchterlony, neutralization, and latex agglutination tests. Thus, we conclude that this clinical isolate of Kanagawa phenomenon-negative V. parahaemolyticus produces a new type of hemolysin that is similar, but not identical, to Vp-TDH, which is usually produced by Kanagawa phenomenon-positive V. parahaemolyticus.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-118926, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-1248866, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-1248868, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-14240539, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-2880146, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-3127502, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-4030087, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-4044797, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-4886581, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-4956587, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-5391048, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-6171517, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-6389667, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-7399675, http://linkedlifedata.com/resource/pubmed/commentcorrection/3126151-7430330
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
961-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin.
pubmed:affiliation
Research Institute for Microbial Diseases, Osaka University, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't