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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-4-1
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pubmed:databankReference |
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pubmed:abstractText |
The spoIIIC locus of Bacillus subtilis has been cloned from the lambda library of Ferrari et al. (E. Ferrari, D. J. HEnner, and J. A. Hoch, J. Bacteriol. 146:430-432, 1981) by using as an assay transformation of the mutant allele spoIIIC94 to the wild type. Regulation of the spoIIIC locus was studied by hybridization of cloned spoIIIC DNA to RNA pulse-labeled at various times during growth and sporulation. The relative rate of transcription of the spoIIIC locus was highest 3 h after the end of growth. The DNA sequence of the spoIIIC transcription unit indicated the coding capacity for a small protein (138 amino acids) having significant similarity with one domain of RNA polymerase sigma factors. Interruption of this coding sequence by an insertion mutation caused cells to become Spo-.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3125151-116131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3125151-12736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3125151-2408275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3125151-2419309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3125151-2863256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3125151-3092189,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3125151-6793556
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
170
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1162-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3125151-Amino Acid Sequence,
pubmed-meshheading:3125151-Bacillus subtilis,
pubmed-meshheading:3125151-Base Sequence,
pubmed-meshheading:3125151-Chromosome Deletion,
pubmed-meshheading:3125151-DNA, Bacterial,
pubmed-meshheading:3125151-Gene Expression Regulation,
pubmed-meshheading:3125151-Genes, Bacterial,
pubmed-meshheading:3125151-Molecular Sequence Data,
pubmed-meshheading:3125151-Mutation,
pubmed-meshheading:3125151-Plasmids,
pubmed-meshheading:3125151-Sigma Factor,
pubmed-meshheading:3125151-Spores, Bacterial,
pubmed-meshheading:3125151-Transcription, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
Transcriptional regulation and structure of the Bacillus subtilis sporulation locus spoIIIC.
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pubmed:affiliation |
Department of Biochemistry, University of Oxford, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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