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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-2-4
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pubmed:abstractText |
A 'serotransferrin-like' protein was purified from mouse milk. This serotransferrin cross-reacts immunologically with the serotransferrin isolated from mouse plasma and not with the mouse lactotransferrin (lactoferrin). Sugar analysis of the three transferrins, i.e. serotransferrin, milk 'serotransferrin-like' protein and lactotransferrin, revealed that the major difference between the glycan primary structure of mouse serotransferrin and those of mouse milk 'serotransferrin-like' protein and lactotransferrin concerns essentially the presence of one fucose residue in the last two proteins. For structural determination, the N-glycosidically linked glycans were released from the protein by a reductive cleavage of the oligosaccharide-protein linkage under strong alkaline conditions. The primary structure of the released oligosaccharide alditols was determined by methylation analysis and 400 MHz 1H-n.m.r. spectroscopy. The oligosaccharide alditols released from milk 'serotransferrin-like' protein and lactotransferrin were identical and were identified as disialylated biantennary glycans of the N-acetyl-lactosamine type with a fucose residue alpha-1,6-linked to the N-acetylglucosamine residue conjugated to the peptide chain and having the following primary structure: NeuAc(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-2)Man(alpha 1-3)[NeuAc(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-2)Man(alpha 1-6)]Man(beta 1-4)GlcNAc(beta 1-4)[Fuc(alpha 1-6)]GlcNAc(beta 1-N)Asn. The serotransferrin glycan has the same primary structure but is only partially fucosylated (10-15%).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-1116600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-1218089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-13772242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-14135466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-14323615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-14423783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-209045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-4042112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-4341488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-4797920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-4998849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-5039936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-5330250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-5677827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-5893780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-5959431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-6226348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-6427010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-7060557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-7083256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-710579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-7144113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-7213791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-7316180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3122730-825141
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
247
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
571-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:3122730-Animals,
pubmed-meshheading:3122730-Carbohydrate Sequence,
pubmed-meshheading:3122730-Chromatography, Liquid,
pubmed-meshheading:3122730-Immunodiffusion,
pubmed-meshheading:3122730-Lactoferrin,
pubmed-meshheading:3122730-Magnetic Resonance Spectroscopy,
pubmed-meshheading:3122730-Methylation,
pubmed-meshheading:3122730-Mice,
pubmed-meshheading:3122730-Milk,
pubmed-meshheading:3122730-Molecular Sequence Data,
pubmed-meshheading:3122730-Oligosaccharides,
pubmed-meshheading:3122730-Peptides,
pubmed-meshheading:3122730-Polysaccharides,
pubmed-meshheading:3122730-Sugar Alcohols,
pubmed-meshheading:3122730-Transferrin
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pubmed:year |
1987
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pubmed:articleTitle |
Primary structure of the glycans from mouse serum and milk transferrins.
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pubmed:affiliation |
Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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