Linked Life Data

3114940

Source:http://linkedlifedata.com/resource/pubmed/id/3114940

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1987-10-20
pubmed:abstractText
We examined the potential of developing a set of species specific and cross reactive monoclonal antibodies (MoAbs) for use in the study of the phylogenetic and functional relation of class I and class II antigens of the major histocompatibility complex (MHC) and leukocyte differentiation antigens in cattle and other species. Comparing immunization strategies demonstrated the number of hybrids producing cross reactive antibodies can be increased by hyperimmunization of mice with lymphoid cells from multiple species. Comparing various methods of assay (antibody-complement mediated cytotoxicity [CT], enzyme linked immunosorbent assay [ELISA] and flow microfluorimetry [FMF]), revealed FMF is the most useful technique for the primary assay of hybridomas producing MoAbs of potential interest. By using dual parameter and dual fluorescence analysis, we could determine whether a given MoAb reacted with mononuclear cells (lymphocytes and monocytes) and/or granulocytes, and also whether any two MoAbs of different isotype and specificity recognized antigens present on identical or separate populations of leukocytes. Comparing the patterns of MoAb reactivity with leukocytes obtained from cows, goats, sheep, pigs, horses and humans, as well as comparing the patterns of reactivity with a panel of lymphoid cell lines derived from cattle (with enzootic bovine leukemia) and humans (with various forms of leukemia), revealed sets of MoAbs reactive with unique antigenic determinants present on BoLA class I (15 MoAbs) and class II (9 MoAbs) antigens, and also MoAbs reactive with determinants present on leukocyte differentiation antigens (36 MoAbs). Dual fluorescence analysis demonstrated the antigens detected by some MoAbs are predominantly expressed on one lineage of leukocytes while others are expressed on two or more lineages of leukocytes. Dual and single fluorescence analysis also demonstrated the PNA receptor(s) is: expressed on T cells, granulocytes and class II antigen monocytes and absent or expressed in low amount on sIgM+ B cells and a newly defined Non T/Non B population of cells. The strategies described for identifying and analyzing the specificity of MoAbs demonstrate the feasibility of developing a set of cross reactive MoAbs for identifying homologous molecules in multiple species and delineating their functional and phylogenetic relation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0165-2427
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
337-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:3114940-Animals, pubmed-meshheading:3114940-Antibodies, Monoclonal, pubmed-meshheading:3114940-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:3114940-Antigens, Surface, pubmed-meshheading:3114940-Cattle, pubmed-meshheading:3114940-Cell Line, pubmed-meshheading:3114940-Cross Reactions, pubmed-meshheading:3114940-Goats, pubmed-meshheading:3114940-Histocompatibility Antigens, pubmed-meshheading:3114940-Horses, pubmed-meshheading:3114940-Humans, pubmed-meshheading:3114940-Leukocytes, pubmed-meshheading:3114940-Lymphocytes, pubmed-meshheading:3114940-Major Histocompatibility Complex, pubmed-meshheading:3114940-Mice, pubmed-meshheading:3114940-Monocytes, pubmed-meshheading:3114940-Rosette Formation, pubmed-meshheading:3114940-Sheep, pubmed-meshheading:3114940-Species Specificity, pubmed-meshheading:3114940-Swine
pubmed:year
1987
pubmed:articleTitle
The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't