Linked Life Data

3089871

Source:http://linkedlifedata.com/resource/pubmed/id/3089871

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-9-16
pubmed:abstractText
Phage Mu d1(Ap,lac) was used to construct the hybrid crp-lac operon in Escherichia coli cells possessing crps mutation which increases sensitivity of bacterial growth to exogenous cAMP. Transcription of the structural lac genes in this hybrid operon was initiated from the promoter region of the crp gene. By measuring levels of beta-galactosidase enzyme, the maximal transcription of the crp gene was obtained in strains with cya or crp mutations causing inactivation of the CRP - cAMP complex. Restoration of cya+crp+ genotype resulted in 5 to 7 times decrease of the lacZ gene activity. At the concentration of exogenous cAMP which induces catabolic derepression in the wild-type strain, the expression of crp-lac operon increased 2-3 times. Using F'ts114lac-mediated chromosome mobilization, the direction of transcription of the crp gene was found to be counterclockwise, relative to the E. coli genetic map.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0016-6758
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
576-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
[Construction of the hybrid crp-lac operon and study of the role of CRP-cAMP complex in its regulation in Escherichia coli].
pubmed:publicationType
Journal Article, English Abstract