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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-5-30
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pubmed:abstractText |
In order to distinguish between the mechanism of microsomal ethanol oxidation and hydroxyl-radical formation, the rate of cytochrome P-450 (P-450)-dependent oxidation of dimethyl sulphoxide (Me2SO) was determined in the presence and in the absence of iron-chelating compounds, in liver microsomes from control, ethanol- and phenobarbital-treated rats. Ethanol treatment resulted in a specific increase (3-fold) of the microsomal ethanol oxidation and NADPH consumption per nmol of P-450. A form of P-450 was purified to apparent homogeneity from the ethanol-treated rats and characterized with respect of amino acid composition and N-terminal amino acid sequence. Specific ethanol induction of a cytochrome P-450 species having a catalytic-centre activity of 20/min for ethanol and consuming 30 nmol of NADPH/min could account for the results observed with microsomes. Phenobarbital treatment caused 50% decrease in the rate of ethanol oxidation and NADPH oxidation per nmol of P-450. The rate of oxidation of the hydroxyl-radical scavenger Me2SO was increased 3-fold by ethanol or phenobarbital treatment when expressed on a per-mg-of-microsomal-protein basis, but the rate of Me2SO oxidation expressed on a per-nmol-of-P-450 basis was unchanged. Addition of iron-chelating agents to the three different types of microsomal preparations caused an 'uncoupling' of the electron-transport chain accompanied by a 4-fold increase of the rate of Me2SO oxidation. It is concluded that ethanol treatment results in the induction of P-450 forms specifically effective in ethanol oxidation and NADPH oxidation, but not in hydroxyl-radical production, as detected by the oxidation of Me2SO.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-1125043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-1147962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-13105648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-168885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-217312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-409715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-4315645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-4690860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-4717531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-5445845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6021815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6091674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6272833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6301163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6327680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6331320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6420404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6427601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6437400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6486823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6497392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6517592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6683096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-6787051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-698185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-7023487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-7085677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-7142188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-7271897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-7424729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3085654-938524
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
233
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
755-61
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3085654-Animals,
pubmed-meshheading:3085654-Cytochrome P-450 Enzyme System,
pubmed-meshheading:3085654-Dimethyl Sulfoxide,
pubmed-meshheading:3085654-Ethanol,
pubmed-meshheading:3085654-Free Radicals,
pubmed-meshheading:3085654-Hydroxides,
pubmed-meshheading:3085654-Male,
pubmed-meshheading:3085654-Microsomes, Liver,
pubmed-meshheading:3085654-NADP,
pubmed-meshheading:3085654-NADPH-Ferrihemoprotein Reductase,
pubmed-meshheading:3085654-Oxidation-Reduction,
pubmed-meshheading:3085654-Phenobarbital,
pubmed-meshheading:3085654-Rats,
pubmed-meshheading:3085654-Rats, Inbred Strains
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pubmed:year |
1986
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pubmed:articleTitle |
Hydroxyl-radical production and ethanol oxidation by liver microsomes isolated from ethanol-treated rats.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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